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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/11/2021 |
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Actualizado : |
01/11/2021 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
FERREIRA, V.; GONZÁLEZ-ARCOS, M.; PIANZZOLA, M.J.; COLL, N.S.; SIRI, M.I.; VALLS, M. |
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Afiliación : |
VIRGINIA FERREIRA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MATIAS GONZÁLEZ-ARCOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JULIA PIANZZOLA, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; NÚRIA S. COLL, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; MARÍA INÉS SIRI, Área Microbiología, Departamento de Biociencias (DEPBIO), Facultad de Química, Universidad de la República, Montevideo, Uruguay; MARC VALLS, Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain; Department of Genetics, University of Barcelona, Bellaterra, Catalonia, Spain. |
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Título : |
Molecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
In: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18 |
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Serie : |
eBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354). |
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ISBN : |
978-1-0716-1608-6 (print) / 978-1-0716-1609-3 (e-book) |
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ISSN : |
1064-3745 (print) / 1940-6029 (electronic) |
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DOI : |
10.1007/978-1-0716-1609-3_18 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online 27 August 2021. |
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Contenido : |
ABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature. |
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Palabras claves : |
Bacterial wilt; Disease resistance; Plant breeding; Potato brown rot; Ralstonia solanacearum; Solanum tuberosum. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02607naa a2200301 a 4500
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008 2021 bl uuuu u00u1 u #d
022 $a1064-3745 (print) / 1940-6029 (electronic)
024 7 $a10.1007/978-1-0716-1609-3_18$2DOI
100 1 $aFERREIRA, V.
245 $aMolecular detection of Ralstonia solanacearum to facilitate breeding for resistance to bacterial wilt in potato.$h[electronic resource]
260 $c2021
490 $aeBook Packages Springer Protocols, (Methods in Molecular Biology, volume 2354).
500 $aArticle history: First Online 27 August 2021.
520 $aABSTRACT. - Potato bacterial wilt is caused by the devastating bacterial pathogen Ralstonia solanacearum. Quantitative resistance to this disease has been and is currently introgressed from a number of wild relatives into cultivated varieties through laborious breeding programs. Here, we present two methods that we have developed to facilitate the screening for resistance to bacterial wilt in potato. The first one uses R. solanacearum reporter strains constitutively expressing the luxCDABE operon or the green fluorescent protein (gfp) to follow pathogen colonization in potato germplasm. Luminescent strains are used for nondestructive live imaging, while fluorescent ones enable precise pathogen visualization inside the plant tissues through confocal microscopy. The second method is a BIO-multiplex-PCR assay that is useful for sensitive and specific detection of viable R. solanacearum (IIB-1) cells in latently infected potato plants. This BIO-multiplex-PCR assay can specifically detect IIB-1 sequevar strains as well as strains belonging to all four R. solanacearum phylotypes and is sensitive enough to detect without DNA extraction ten bacterial cells per mL in complex samples. The described methods allow the detection of latent infections in roots and stems of asymptomatic plants and were shown to be efficient tools to assist potato breeding programs. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
653 $aBacterial wilt
653 $aDisease resistance
653 $aPlant breeding
653 $aPotato brown rot
653 $aRalstonia solanacearum
653 $aSolanum tuberosum
700 1 $aGONZÁLEZ-ARCOS, M.
700 1 $aPIANZZOLA, M.J.
700 1 $aCOLL, N.S.
700 1 $aSIRI, M.I.
700 1 $aVALLS, M.
773 $tIn: Dobnik D., Gruden K., Ram?ak ?., Coll A. (eds). Solanum tuberosum. Methods in Molecular Biology, 2021, vol 2354. Humana, New York, NY. doi: https://doi.org/10.1007/978-1-0716-1609-3_18
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
07/11/2022 |
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Actualizado : |
07/11/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
HYDE, K.A.; AGUIAR, F.L.N.; ALVARENGA, P.B.; REZENDE, A.L.; ALVES, B.G.; ALVES, K.A.; GASTAL, G.D.A.; GASTAL, M.O.; GASTAL, E.L. |
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Afiliación : |
KENDALL A. HYDE, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; FRANCISCO L.N. AGUIAR, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Paraíba, Sousa, Brazil; PAULA B. ALVARENGA, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; AMANDA L. REZENDE, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; BENNER G. ALVES, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; KELE A. ALVES, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; MELBA O. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
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Título : |
Characterization of preantral follicle clustering and neighborhood patterns in the equine ovary. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
PLoS ONE, 2022, Volume 17, Issue 10, e0275396. OPEN ACCESS. doi: https://doi.org/10.1371/journal.pone.0275396 |
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ISSN : |
1932-6203 |
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DOI : |
10.1371/journal.pone.0275396 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received June 10 2022; Accepted September 14 2022; Published October 4 2022. Editor: Meijia Zhang, China Agricultural University, CHINA. -- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/ ) -- |
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Contenido : |
ABSTRACT. - Understanding the transition from quiescent primordial follicles to activated primary follicles is vital for characterizing ovarian folliculogenesis and improving assisted reproductive techniques. To date, no study has investigated preantral follicle crowding in the ovaries of livestock or characterized these crowds according to follicular morphology and ovarian location (portions and regions) in any species. Therefore, the present study aimed to assess the crowding (clustering and neighborhood) patterns of preantral follicles in the equine ovary according to mare age, follicular morphology and developmental stage, and spatial location in the ovary.
© 2022 Hyde et al. T |
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Palabras claves : |
Animals; Cluster Analysis; Horses; Ovarian Follicle; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16827/1/journal.pone.0275396.pdf
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0275396
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Marc : |
LEADER 02089naa a2200313 a 4500
001 1063706
005 2022-11-07
008 2022 bl uuuu u00u1 u #d
022 $a1932-6203
024 7 $a10.1371/journal.pone.0275396$2DOI
100 1 $aHYDE, K.A.
245 $aCharacterization of preantral follicle clustering and neighborhood patterns in the equine ovary.$h[electronic resource]
260 $c2022
500 $aArticle history: Received June 10 2022; Accepted September 14 2022; Published October 4 2022. Editor: Meijia Zhang, China Agricultural University, CHINA. -- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. (http://creativecommons.org/licenses/by/4.0/ ) --
520 $aABSTRACT. - Understanding the transition from quiescent primordial follicles to activated primary follicles is vital for characterizing ovarian folliculogenesis and improving assisted reproductive techniques. To date, no study has investigated preantral follicle crowding in the ovaries of livestock or characterized these crowds according to follicular morphology and ovarian location (portions and regions) in any species. Therefore, the present study aimed to assess the crowding (clustering and neighborhood) patterns of preantral follicles in the equine ovary according to mare age, follicular morphology and developmental stage, and spatial location in the ovary. © 2022 Hyde et al. T
653 $aAnimals
653 $aCluster Analysis
653 $aHorses
653 $aOvarian Follicle
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
700 1 $aAGUIAR, F.L.N.
700 1 $aALVARENGA, P.B.
700 1 $aREZENDE, A.L.
700 1 $aALVES, B.G.
700 1 $aALVES, K.A.
700 1 $aGASTAL, G.D.A.
700 1 $aGASTAL, M.O.
700 1 $aGASTAL, E.L.
773 $tPLoS ONE, 2022, Volume 17, Issue 10, e0275396. OPEN ACCESS. doi: https://doi.org/10.1371/journal.pone.0275396
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